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Thank you. Great tutorial.

.TNF-alpha= 450 value - 0.132 Concentration - 11.792 Inme se result konsa hai.. please tell me

I need to prepare a presentation about ELISA and your videos are saving my ass. Ty

Hi is this applicable to direct ELISA too?

Thank you!

Does Competitive ELISA first coat antigen to well plate or antibody?

10 years later and this is still the best explanation I have found. Thank You!

Million thanks! I found this video very helpful

Great Video!!!!

This is great. Simple explanation. Thank you so much!

prism 10 hack macbook

Great thanks. From Pakistan

❤❤

As an educator and a researcher, I just want to take a moment to appreciate how amazing you are with your explanations. Clear, concise, comprehensive. Simply amazing! Thank you so much for sharing your expertise 🤗

Thank you very much! You just can`t imagine how this video helped me!

I get that I am very late to the party with my comment. But can I just point out that our hero here woke up before 8 on a sunday, just to make a video of graphpad. Now thats what I call enthusiasm!

very well described 👍👍👍👍

This video was very very helpful ! Thank you !!

Thanks, help a lot!

Sir, you are a lifesaver

Man, you are the man. thanks for your help. Hi From Mexico

Helpful

all fine until you plugged in 1/abs to x values.. can you explain that a little..

How long did it take ?

where is the 3rd part? is it still coming?

I have IDEXX ELISA kit for paratuberculosis. Formula is given there on brochure. OD values have to be incorporated in formula. But end results are confusing. I need help please.

Egad! What manner of Vacuuming Witchcraft be this?!

where is the link to download Graph pad prism?i could not find it

Well. I couldn't find 90-well Plate Layout. There was no "print-outs" on your website.

Does It run on mac?

happy happy happy (voz de pito)...

Very useful! many thanks!

Really helpful thank you very much

What to do if curve fit equation has E

That was really helpful. Thank you so much! The problem I am experiencing is that Prism would just "make up" values for the readings that were negative or 0. Even though in one run I had a zero standard given with the absorbance and concentration of zero it would calculate a concentration of 50 for another sample that had the same absorbance?! Any suggestions on how I can prevent this, so that my interpolation will also just not give me any result for the samples at or below 0?

Thank you so much. You explained in simple and lucid way.

Dr. Hammonds: Thanks, you took a complex idea and made it much more simple and easier to understand.

Which protein concentration do you load? 10ng or 20ng of whole cell lysate?

You could have also shown us how to calculate intra-assay and inter-assay coefficient of variations

Thank you for the tutorial. I use graph pad Prism 8. Please help me as my Table of Results is missing so I cannot determine R2

perfect explanation man, keep going👌

best video i have seen on elisa

Great job

Very interesting

Wow! thanks so much. This is very helpful. I will appreciate more tutorials on graphpad

if I have three groups, I want to compare their weight at three different time points, which type of graph will use, please

if I have three groups, I want to compare their weight at three different time points, which type of graph will use, please

Thanks very much!I was really struggling with it but you made my life simpler

thank you for this video. you made ELISA assay easy.

Thanks a lot for your help, is so importan to my thesis